BR Biochem Life Sciences Private Limited

BR-GELD-2100 Series Gel Documentation and Analysis System is designed for the detection and documentation of nucleic acids and proteins. It adopts a high resolution and high sensitive scientific CCD-Camera, which enables the instrument to capture utter weak signals under extremely low illumination condition. The automatic controls and the intuitive software we offered can help the researchers to get rid of the complicated operation process and increase therefore the experiment efficiency.

Gel Inspection: Diverse dyes,  e.g.: Ethiduim Bromide, SYBRGold, SYBR Green, SYBRSafe, GelStar, Texas Red, Fluorescein, marked DNA/RNA detection.
 
Protein Inspection: Commassie Brilliant Blue, Silver Staining and Fluorescent dyes, e.g.: Sypro Red,  SyproOrange???Deep Purple, Pro-Q Diamond etc.
 
Others: Others:  ELISA Plate, Petri Dish Colony Counter, TLC Plate, etc.

Total RNA Mini Kit (Tissue) RT050, RT100, RT300

The Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue and paraffin-embedded tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. TheTotal RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Total RNA Mini Kit (Blood/Cultured Cell) RB050, RB100, RB300

The RNA Kit (Blood/Cultured Cell) is a RNA Isolation Kit designed for total RNA purification from fresh whole human blood and cultured cells. ThisRNA Kit will purify RNA from blood within 20 minutes using an efficient RNA miniprep system. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column and once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water and ready for use in various downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Total RNA Mini Kit (Plant) RP050, RP100, RP300

plant species within 15 minutes. Samples are initially ground in liquid nitrogen and then filtered to remove cell debris using the efficient RNA filter columns. In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fiber matrix of the RNA spin column. Once any contaminants have been removed using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified total RNA is ready for use in RT-PCR, Real-time PCR and Northern Blotting. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Taq DNA Polymerase is thermostable 5 3 DNA polymerase. It lacks 3 5 exonuclease (proofreading) activity and has low 5´ 3´ exonuclease activity. Polymerase exhibits deoxynucleotidyl transferase activity resulting in A-overhang at the 3‘-ends of PCR products and allowing for TA cloning. The PCR accuracy of Taq DNA Polymerase is 4.5 x 104 (a number of nucleotides incorporated before the first error occurs).

Applications:

• RT-PCR, Colony PCR
• TA cloning, library construction

Benefits:

• High yields in routine PCR, good performance in fast PCR

Specifications:

• Category: PCE0202
• Size: 3000 unit
• Component: 2 x 1500 u - Taq DNA Polymerase, 5 u/µl 8 x 2 ml - 10X PCR Buffer 4 x 2 ml - 50 mM MgCl2
• Component composition: Enzyme in storage buffer. 10X PCR Buffer contain enhancers and stabilizers, but no dNTPs and no Mg2+.