BR Biochem Life Sciences Private Limited

Total RNA Mini Kit (Tissue) RT050, RT100, RT300

The Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue and paraffin-embedded tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. TheTotal RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Total RNA Mini Kit (Blood/Cultured Cell) RB050, RB100, RB300

The RNA Kit (Blood/Cultured Cell) is a RNA Isolation Kit designed for total RNA purification from fresh whole human blood and cultured cells. ThisRNA Kit will purify RNA from blood within 20 minutes using an efficient RNA miniprep system. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column and once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water and ready for use in various downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Total RNA Mini Kit (Plant) RP050, RP100, RP300

plant species within 15 minutes. Samples are initially ground in liquid nitrogen and then filtered to remove cell debris using the efficient RNA filter columns. In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fiber matrix of the RNA spin column. Once any contaminants have been removed using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified total RNA is ready for use in RT-PCR, Real-time PCR and Northern Blotting. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Taq DNA Polymerase is thermostable 5 3 DNA polymerase. It lacks 3 5 exonuclease (proofreading) activity and has low 5´ 3´ exonuclease activity. Polymerase exhibits deoxynucleotidyl transferase activity resulting in A-overhang at the 3‘-ends of PCR products and allowing for TA cloning. The PCR accuracy of Taq DNA Polymerase is 4.5 x 104 (a number of nucleotides incorporated before the first error occurs).

Applications:

• RT-PCR, Colony PCR
• TA cloning, library construction

Benefits:

• High yields in routine PCR, good performance in fast PCR

Specifications:

• Category: PCE0202
• Size: 3000 unit
• Component: 2 x 1500 u - Taq DNA Polymerase, 5 u/µl 8 x 2 ml - 10X PCR Buffer 4 x 2 ml - 50 mM MgCl2
• Component composition: Enzyme in storage buffer. 10X PCR Buffer contain enhancers and stabilizers, but no dNTPs and no Mg2+.

Presto™ Mini gDNA Bacteria Kit GBB100

The Presto™ Mini gDNA Bacteria Kit is a Bacteria genomic DNA extraction kit optimized for genomic and viral DNA purification from Gram negative bacterial cells and Gram positive bacterial cells. This Bacteria Genomic DNA Extraction Kit includes Gram+ Buffer and Lysozyme and when combined, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 30 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA, with approximately 20-40 kb, is suitable for use in PCR or other enzymatic reactions.

Presto™ Mini gDNA Bacteria Kit Functional Test Data (Gram (-) Negative Bacteria

Figure 1. Genomic DNA (approximately 30 kb) was extracted using the Presto™ Mini Bacteria Kit. An Escherichia coli (1×10⁹⁾ culture (OD600=2, 1 ml) was harvested by centrifugation at 16,000 x g for 1 minute. A 5 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder

Specifications:

Sample	Yield	260/280	260/230	Qty per case
Test 1	38.16 µg	2.05	2.26	1
Test 2	33.88 µg	2.05	2.27	1
Test 3	39.02 µg	2.07	2.29	1
SDU200	200 L	12	150 DN	1.5
SDU300	300 L	16	225 DN	2.5

Genomic DNA Mini Kit (Plant) GP100

Genomic DNA Mini Kit (Plant) GP100The Genomic DNA Mini Kit (Plant) is a genomic DNA Extraction Kit optimized for genomic, mitochondrial and chloroplast DNA purification from plant tissue. This DNA Extraction Kit processes plant samples by grinding in liquid nitrogen followed by lysis buffer incubation. The lysate is treated with RNase A then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. DNA phenol extraction or alcohol precipitation is not required, and the entire procedure can be completed in less than 30 minutes.
100 Prep